Measures TSB concentration in 5 seconds Measures undiluted serum Corrects for oxyhemoglobin Full measuring range

Reichert's UNISTAT® Bilirubinometer uses a photometric analyzer to provide accurate and rapid measurements of total serum bilirubin (TSB) concentration in undiluted serum. It is an instrument providing point-of-care testing for all skin types and ethnicities. The photometric analyzer is the ideal STAT-level analyzer for determining TSB in newborns.

The UNISTAT® Bilirubinometer is the most trusted and reliable STAT analysis of TSB available. The results from a recent 3rd party study determined that the correlation between the Reichert UNISTAT® and the diazo Jendrassik-Grof method was 99%.¹

Principles of Operation

The Reichert UNISTAT® is a direct spectrophotometer that measures total bilirubin in neonatal serum samples. Dilution is not necessary. A beam of light is passed through the sample cuvette. The beam is split by a dichroic mirror and directed toward the 460nm and 550nm filters. The transmittance of the light exiting the filters is measured by photodetectors.

The control electronics calculate total bilirubin based on output from the photodetectors. Since peak absorbance is shared by bilirubin and oxyhemoglobin at 460nm, it is necessary to measure oxyhemoglobin at 550nm. The difference in the readings at 460nm and 550nm is the total bilirubin value, corrected for the presence of oxyhemoglobin. Since the UNISTAT® measures and subtracts oxyhemoglobin, bilirubin results are not affected by hemolyzed samples.
  Figure 1 chart shows the extremely high correlation of measurements taken by the UNISTAT® Bilirubinometer compared against the same patient samples measured by a clinical hospital analyzer using the diazo Jendrassik-Grof method. This method is a widely recognized diazo method for Total Serum Bilirubin measurement. The UNISTAT® demonstrated a correlation of 99% with the diazo Jendrassik-Grof method. This is important for the physician’s office and satellite location to obtain accurate measurements that correlate to the hospital.
  Figure 2 chart shows agreement between the two methods (UNISTAT® and diazo Jendrassik-Grof) using a Bland-Altman plot analysis. Bland-Altman plots in analytical chemistry and biostatistics is a method of data plotting used in analyzing the agreement between two different assays.