Programmable Slide Moat, 12 Slide Capacity, 230V
The Boekel Scientific programmable slide hybridization system offers researchers a compact 12 position Slide Hybridization system capable of performing complex IHC and InSitu Hybridization reactions. The digital microprocessor control offers full programmability of up to three time controlled temperature set points. The precision temperature control and sealed chamber offer the ideal environment for controlled IHC and InSitu Hybridization reactions. The Boekel Scientific Programmable Slide Hybridization system offers greater functionality with a compact footprint that compliments our complete line of Hybridization and Slide Hybridization systems.
Applications
- In situ hybridization
- All single temperature microscope slide incubation procedures
- Slide warming and drying
- Immuno-histochemistry
- FISH
- Histology, Hematology
FISH Overview
Molecular diagnostics is the fastest growing segment of the diagnostics market. Fluorescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of:
- Prenatal diagnosis
- Cancer diagnosis
- Molecular cytogenetic of birth defects and mental retardation
- Chromosomal abnormalities
- Clinical genetics
- Neuroscience
- Reproductive medicine
- Toxicology
- Microbial ecology
- Evolutionary biology
- Comparative genomics
- Cellular genomics
- Differentiation between benign and malignant tumors
The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined.
The equipment necessary is as follows:
- Balance
- Stir plate
- Micro-centrifuge
- Refrigerator
- Freezer
Tissue preparation will require:
Performing the FISH procedure will require the following equipment and materials in addition to reagents:
- Slide dryer
- Water baths
- Hybridization Oven
- Coplin jars
- Coverslips
- Staining dishes
- Rubber cement (Optional: depends on equipment used)
- Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography