Slide Moat Slide Hybridizer, 115V
The Slide Moat Slide Hybridizer from Boekel Scientific is a winner for FISH and other lab hybridization procedures. With a capacity for 30 standard microscopic slides and excellent temperature stability and uniformity, this high-quality slide moat positions your team for success.
The tempered glass lid seals with an integral gasket on the heating base, and an optional light shield is available for photosensitive reagents.
Applications
- In situ hybridization
- All single temperature microscope slide incubation procedures
- Slide warming and drying
- Immuno-histochemistry
- FISH
- Histology, Hematology
Unit Overview
The Boekel Slide Moat is designed to provide a heating platform and chamber capable of maintaining either dry or highly humid conditions for In-Situ Hybridization applications. Uses include in-situ RNA amplification, reverse transcription reactions, hybridization and DNA melt. The Boekel Slide Moat can hold up to 30 standard microscope slides. The unit has an advanced PID temperature controller and high density heating plate for providing very stable and accurate temperatures from ambient plus 5˚C to 100˚C. The unit also provides eight humidity generation wells which can be used to create a humid environment for 12 or more hours. The unit comes with a see-through tempered glass lid for observing reactions and verifying humidity conditions. There is sufficient clearance (13/32”/1cm) between the heat plate and the glass lid bottom to allow for the use of most in-situ cell carriers and for adding a flat surface temperature gage to verify slide temperature. Note: Boekel strongly recommends using covered and sealed slides for higher temperatures (> 37° C) or prolonged period incubations (> 1 hour).
FISH Overview
Molecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of:
- Prenatal diagnosis
- Cancer diagnosis
- Molecular cytogenetic of birth defects and mental retardation
- Chromosomal abnormalities
- Clinical genetics
- Neuroscience
- Reproductive medicine
- Toxicology
- Microbial ecology
- Evolutionary biology
- Comparative genomics
- Cellular genomics
- Differentiation between benign and malignant tumors
The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined.
The equipment necessary is as follows:
- pH meter
- Balance
- Stir plate
- Micro-centrifuge
- Refrigerator
- Freezer
Tissue preparation will require:
Performing the FISH procedure will require the following equipment and materials in addition to reagents:
- Slide dryer
- Water baths
- Hybridization Oven
- Coplin jars
- Coverslips
- Staining dishes
- Rubber cement (Optional: depends on equipment used)
- Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography