These bioreactor flasks are viable in vitro alternatives to using mouse ascites, standard cell culture, or tissue culture products. Bioreactors utilize innovative membrane technology to separate the cell cultivation area from the media chamber. Cells and cellular-secreted products with an MWCO over 10kD are retained between two non-cytotoxic membranes. The upper semi-permeable membrane allows continuous nutrient diffusion and waste elimination. The lower membrane provides direct oxygenation and ensures optimum gas exchange. Separate ports give access to either media or cell cultivation chamber allowing easy change of expended medium without interference of cell growth, function, or gas exchange.
Ideal for monoclonal antibody production up to 5mg/mL, recombinant protein expression, and concentrated cell densities of 108 cell/mL. Allows production of up to 1 x 109 cells per cell compartment. The 15 mL cultivation chamber is ideal for small production runs. A 1000 mL media compartment has vertical and horizontal volume markings from 100-1000mL. Produces about 80mg/month of antibody.