• Optimized for efficient probe-based detection of multiple targets in a single reaction well
• Two-tube formulation: 2x Probe One-Step Mix and 20x RTase Blend (Includes Hot Start Taq Polymerase)
• Convenient cDNA synthesis and subsequent qPCR in a single tube
• High-purity enzyme formulation for enhanced stability and performance
• Ideal for gene expression analysis & multiple pathogen detection

The Accuris PR2122 qMAX Probe One-Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory.

**Sample size is for product evaluation. Only one sample per item can be purchased**

qMAX Probe One-Step Multiplex kits are designed to overcome the challenges of multiplex RT-PCR by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

Each reaction kit includes:

• 20x thermostable MMLV derived RTase (RNase inhibitor included)
• 2x Probe One-Step Mix
• Includes Accuris Hot Start Taq DNA Polymerase
• dNTPs
• Optimized buffer for maximum efficiency and sensitivity

*Reaction pack size dependent on the ordered option

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments. Three versions of the One Step qPCR kits are available:

qMAX Green One Step kits [PR2120] incorporate Accuris’ proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits [PR2121] are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits [PR2122] are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.