• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• Hot-Start Taq allows for preparation at room-temperature
• Intercalating dye exhibits higher fluorescent and lower PCR inhibition
• Ideal for cDNA, gDNA, and low-copy number templates

The Accuris PR2120 qMAX Green One-Step RT-qPCR kit incorporates Accuris’ intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR. qMAX one-step kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

**Sample size is for product evaluation. Only one sample per item can be purchased**

Each reaction kit includes:

• 20X thermostable MMLV derived RTase (RNase inhibitor included)
• 2X Green One-Step Mix
• Includes Accuris Hot Start Taq DNA Polymerase
• qMAX Green (proprietary fluorescent dye)
• dNTPs
• Optimized buffer for maximum efficiency & sensitivity

*Reaction pack size dependent on ordered option 

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments. Three versions of the One Step qPCR kits are available:

qMAX Green One Step kits [PR2120] incorporate Accuris’ proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits [PR2121] are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits [PR2122] are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.