• Convenient cDNA synthesis and PCR in a single tube from 1pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room-temperature
• ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels
• Sample size for product evaluation; only one sample can be purchased 

The Accuris PR1100 One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end point analysis. This RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and Accuris Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The Accuris One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

Mouse Total RNA starting material was used with the Accuris One-Ste[ RT-PCR kit to produce 1Kb fragment with RN18S primers. A serial dilution shows relative quantities and excellent specificity of DNA form 100pg, 10pg, and 1pg input RNA.

**Please note, sample sizes are for product evaluation. Only one sample size per item per order can be purchased.