The Accuris One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for endpoint analysis. The RT-PCR Kit consists of MMLV-derived Thermostable Reverse Transcriptase, a potent RNase Inhibitor, and Accuris HS Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material. A highly optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (GC-rich targets). The Accuris OneStep RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels.