• Proprietary 5X reaction buffer includes enhancers for maximizing enzyme activity and reaction speed
• Performs across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences
• Bound with a monoclonal antibody that blocks enzyme activity allowing reactions to be set up at room temperature
• Includes 5X buffer; 2X Master Mix available (sold separately); can be ordered with or without a red gel loading dye - See PR1001-HS / PR1001-HSR
• NOTE: Sample size is intended for evaluation; only one sample size can be purchased

Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification. When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction.

Hot Start Taq is provided with a 5X buffer. Alternatively, it is available in a ready to use 2X Master Mix (sold separately - see PR1001-HS). The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

Gel image shows exceptional sensitivity of Accuris Hot Start Taq Polymerase with 405bp plant mitochondrial DNA. Amplification was performed with decreasing amounts of starting material, from 500ng to 31.25ng.

** Please note, sample size is intended for product evaluation. Only one sample can be purchased**